Hi James,
I did check teh B-factors and they are similar to the flanking regions (about
40). The difference density appeared at the later stage of refinement (TLS and
restrained in CCP4i).
What do you think and how to do it?
Best,
Sun Tang
James Irving <[EMAIL PROTECTED]> wrote: Hi Sun,
I suggest checking that the b-factors for those residues aren't
unexpectedly high compared to those in the flankinh regions.
Cheers,
James
On 1/23/08, Sun Tang wrote:
> Hello Everyone,
>
> When I refined a structure, I found strong difference density Fo-Fc at 3
> sigma contour for the for five residues which already have occupancy of 1.
> The density is continuous and so strong as if I did not put the residues
> there. Why was that? Can I put greater than 1 occupancy for those residues?
>
> Thank you very much for your opinions and suggestions!
>
> Best wishes,
>
> Sun Tang
>
>
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Dr. James Irving
NH&MRC C.J. Martin Fellow
School of Biomedical Sciences
Building 13D
Monash University
Wellington Road
Melbourne 3800
Australia
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