Hi Margriet It's almost certainly due to diffuse scattering as a result of correlated atomic displacements. See this: http://www.nature.com/nsmb/journal/v1/n2/pdf/nsb0294-124.pdf .
Are they lines or sheets, in other words do they appear only on one image, or are they also on adjacent images, i.e. are you looking at a slice through more extensive diffuse scattering? According to the above paper there are at least 3 types of DS: haloes around each Bragg spot (thermal diffuse or acoustic scattering) due to long range displacements correlated over different unit cells (this is very common); DS located along reciprocal lattice planes which results from anisotropic intermolecular displacements correlated over a few unit cells (which is I think what you are seeing), and low-intensity very diffuse background patches (optic scattering), but I don't see much of that. -- Ian > -----Original Message----- > From: owner-ccp...@jiscmail.ac.uk > [mailto:owner-ccp...@jiscmail.ac.uk] On Behalf Of Margriet Ovaere > Sent: 28 January 2009 13:52 > To: ccp...@dl.ac.uk > Subject: small lines in diffraction pattern > > Dear all, > > In the diffraction pattern of crystals of an RNA decamer, > small lines appeared (see pictures attached). We've tried > different crystals but they all showed the same small lines. > Has anybody seen thisphenomenabefore and has got an > explanation for it please..? > > Many thanks > > Margriet Ovaere > > > Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing i.tic...@astex-therapeutics.com and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof. Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, Cambridge CB4 0QA under number 3751674
