Re: [ccp4bb] small lines in diffraction patternActa Cryst. (1998). D54, 848-853 
   [ doi:10.1107/S0907444998001875 ]

A Description of Imperfections in Protein Crystals
C. Nave
Abstract: An analysis is given of the contribution of various crystal 
imperfections to the rocking widths of reflections and the divergence of the 
diffracted beams. The crystal imperfections are the angular spread of the 
mosaic blocks in the crystal, the size of the mosaic blocks and the variation 
in cell dimensions between blocks. The analysis has implications for improving 
crystal perfection, defining data-collection requirements and for 
data-processing procedures. Measurements on crystals of tetragonal lysozyme at 
room temperature and 100 K were made in order to illustrate how parameters 
describing the crystal imperfections can be obtained. At 100 K, the dominant 
imperfection appeared to be a variation in unit-cell dimensions in the crystal.


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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************

----- Original Message ----- 
From: Jacob Keller 
To: CCP4BB@JISCMAIL.AC.UK 
Sent: Wednesday, January 28, 2009 11:57 AM
Subject: Re: [ccp4bb] small lines in diffraction pattern


I had thought that in a previous thread, we had all come to a consensus that 
actually the largest source of what is normally explained as "mosaicity" is 
really differences in unit cell size, due perhaps to uneven shrinkage in 
crystals upon freezing or otherwise. I believe that there was actually an acta 
cryst paper which investigated all of the various ingredients of "mosaicity" 
which supports this (this is why I said it.)

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************

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