Jacob Traditional mosaic spread (ordered mosaic blocks imperfectly aligned with respect to one another) gives spherical caps in reciprocal space. These would appear as arcs on a single crystal rotation photograph. If anisotropic, the arcs would be more extensive in some directions. The patterns do not appear to fit this case. Different integration software could have different models for "mosaicity" but they often simply ensure that most of the intensity is captured in the integration volume by choosing some appropriate reflection range for the reflection. This could capture broadening of the reflections due to many types of disorder, including some of the ones discussed by Ian and myself. However, for the case discussed here, I think most integration programs would struggle. Regards Colin
________________________________ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob Keller Sent: 28 January 2009 17:05 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] small lines in diffraction pattern Couldn't the lines be explained most simply by extreme mosaicity, perhaps severely anisotropic? If not, why not? What were the values obtained in integration? Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu ******************************************* ----- Original Message ----- From: Margriet Ovaere <mailto:margriet.ova...@chem.kuleuven.be> To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, January 28, 2009 7:51 AM Subject: [ccp4bb] small lines in diffraction pattern Dear all, In the diffraction pattern of crystals of an RNA decamer, small lines appeared (see pictures attached). We've tried different crystals but they all showed the same small lines. �Has anybody seen this�phenomena�before and has got an explanation for it please..? Many thanks Margriet Ovaere ________________________________ ________________________________ Margriet Ovaere Chemistry Department K.U.Leuven Biomolecular Architecture Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 ________________________________ Dear all, In the diffraction pattern of crystals of an RNA decamer, small lines appeared (see pictures attached). We've tried different crystals but they all showed the same small lines. Has anybody seen this phenomena before and has got an explanation for it please..? Many thanks Margriet Ovaere  Margriet Ovaere Chemistry Department K.U.Leuven Biomolecular Architecture Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm <DIV><FONT size="1" color="gray">This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom </FONT></DIV>
