Have a look at REFOLD: http://refold.med.monash.edu.au/
cheers
Ashley
On 05/05/2009, at 9:27 PM, Sanjiv Kumar wrote:
I am working on a membrane protein. There was problem with the
normal purification of the protein so I have tired to purify it
under denaturing conditions (Using 8M Urea). Luckily I could purify
the protein in large quantity. But now the problem is that I am not
able to refold the protein by step wise removing 8M Urea by dialysis
(8M, 6M, 4M, 2M, 1M, 0.5M and 0M urea). It is showing aggregation at
very high urea concentration say 2M urea. Kindly suggest any
alternate method that I can try for refolding of this protein. It is
a prokaryotic protein, cloned in pET28a and expressed in BL21 DE3.
Please Help.
Sanjiv Kumar
Associate Professor Ashley M Buckle
NHMRC Senior Research Fellow
The Department of Biochemistry and Molecular Biology
School of Biomedical Sciences, Faculty of Medicine &
Victorian Bioinformatics Consortium (VBC)
Monash University, Clayton, Vic 3800
Australia
http://www.med.monash.edu.au/biochem/staff/abuckle.html
iChat/AIM: blindcaptaincat
skype: ashley.buckle
Tel: (613) 9902 9313 (office)
Fax : (613) 9905 4699
