My post-doc recently produced a splendid (for its resolution) ~5A map of a "medium" sized protein-DNA complex using Ta6Br12 clusters. And he's got a good toehold on a ~340kDa complex using the same clusters. So I'm recently converted to these little nuggets. Phoebe
---- Original message ---- >Date: Thu, 14 May 2009 09:35:28 +0200 >From: Clemens Grimm <clemens.gr...@biozentrum.uni-wuerzburg.de> >Subject: [ccp4bb] Phasing at Low Resolution >To: CCP4BB@JISCMAIL.AC.UK > >Dear all, > >after the SeMet phasing discussion, what would be -in general- the >best technique to phase low resolution data (<=4A) of large complexes >(>=150 kDA) - in terms of > > - derivatization compounds (is there something like the 'golden >five' HA compounds for these cases), > >- data collection techniques and > >- phasing methods? > >Clemens Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 RNA is really nifty DNA is over fifty We have put them both in one book Please do take a really good look http://www.rsc.org/shop/books/2008/9780854042722.asp
