I like PIP (di-mu-iodo-bis-ethylenediamine-di-platinum (II) nitrate).
At low resolution it offers you 4 heavy atoms (I2Pt2), a choice of
anomalous signal, and at higher resolution if you can resolve the 4
heavy atoms you then instantly get higher resolution phases.
It likes binding as a low-res ball in active sites and with more order
at -S-S- (disulfide) and -S- (methionine thioether) positions. I've also
seen it rather arbitrarily located in a solvent channel
Cheers,
Charlie
Phoebe Rice wrote:
My post-doc recently produced a splendid (for its resolution)
~5A map of a "medium" sized protein-DNA complex using Ta6Br12
clusters. And he's got a good toehold on a ~340kDa complex
using the same clusters. So I'm recently converted to these
little nuggets.
Phoebe
---- Original message ----
Date: Thu, 14 May 2009 09:35:28 +0200
From: Clemens Grimm <clemens.gr...@biozentrum.uni-wuerzburg.de>
Subject: [ccp4bb] Phasing at Low Resolution
To: CCP4BB@JISCMAIL.AC.UK
Dear all,
after the SeMet phasing discussion, what would be -in
general- the
best technique to phase low resolution data (<=4A) of large
complexes
(>=150 kDA) - in terms of
- derivatization compounds (is there something like the
'golden
five' HA compounds for these cases),
- data collection techniques and
- phasing methods?
Clemens
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
RNA is really nifty
DNA is over fifty
We have put them
both in one book
Please do take a
really good look
http://www.rsc.org/shop/books/2008/9780854042722.asp
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
charles.b...@uwa.edu.au
+61 8 6488 4406
