Dear Min, You can use microscale thermophoresis for this kind of tricky cases when you have limited amount of protein and ligands. There is enough literature on this topic.
have a look in the following reference: Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19678-82 Hope this helps. Regards, Krishna Krishna Chinthalapudi Graduate Student Hannover Medical School Germany On Thu, Aug 4, 2011 at 4:32 AM, m zhang <mzhang...@hotmail.com> wrote: > Dear all, > > I have two unrelated questions. Any suggestion on any of them will be > greatly appreciated. > > First, we have two proteins that bind each other without a doubt. But since > we have very limited amount of proteins and it takes a long time to > reproduce them, we are very hesitating to try ITC and AUC to find out the > stoichiometry of these complex. So I am wondering if there is any other way > we can try to find the ratio without consuming much proteins? > > Second, we are thinking about getting a new incubator for crystallization. > Could anyone here recommend a model or a brand? > > Thank you, > > Min > >
