Dear all, Thank you all very much for your kind suggestions. Now we have some
preliminary data of SPR. But we might try other new ideas suggested here.
Best,Min
Date: Thu, 4 Aug 2011 13:19:41 +0200
From: krishna....@gmail.com
Subject: Re: [ccp4bb] stoichiometry of complex and incubator
To: CCP4BB@JISCMAIL.AC.UK
Dear Min,
You can use microscale thermophoresis for this kind of tricky cases when you
have limited amount of protein and ligands. There is enough literature on this
topic.
have a look in the following reference:
Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19678-82
Hope this helps.
Regards,
Krishna
Krishna Chinthalapudi
Graduate Student
Hannover Medical School
Germany
On Thu, Aug 4, 2011 at 4:32 AM, m zhang <mzhang...@hotmail.com> wrote:
Dear all,
I have two unrelated questions. Any suggestion on any of them will be greatly
appreciated.
First, we have two proteins that bind each other without a doubt. But since we
have very limited amount of proteins and it takes a long time to reproduce
them, we are very hesitating to try ITC and AUC to find out the stoichiometry
of these complex. So I am wondering if there is any other way we can try to
find the ratio without consuming much proteins?
Second, we are thinking about getting a new incubator for crystallization.
Could anyone here recommend a model or a brand?
Thank you,
Min
