I'm pretty certain that Jalview http://www.jalview.org/ can do that (you might have to set the user defined colours yourself).
Dave. ============================ David C. Briggs PhD Father, Structural Biologist and Sceptic ============================ University of Manchester E-mail: david.c.bri...@manchester.ac.uk ============================ Webs : http://flavors.me/xtaldave Twitter: @xtaldave Skype: DocDCB ============================ On 3 February 2012 05:32, WENHE ZHONG <wenhezhong.xmu....@gmail.com> wrote: > Dear members, > > Apologize for this off-topic question. I am looking for a protein sequence > alignment tool which is capable to generate a particular output file similar > to the attached format (please see the attached picture). I have been > looking at some popular programs but none of them can show the conserved > amino acids by colored blocks as shown in the attached file. > > Maybe some of you have seen some programs can do this? Thank you. > > King regards, > Wenhe