This is in response to a comment to this thread Kevin, Could you explain how that worked? How do you know your method worked? Did you estimate the lipopolysaccharide before and after the method?
The method already mentioned here to wash using TritonX100 makes sense. by washing bound protein sample May able to phase separate into detergent micellar phase and get rid of endotoxins. Most widely accepted method to separate is by two phase micellar system. above the CMC endotoxins will be accomodated by micelles through non-polar interactions of alkyl chains of lipidA. Padayatti PS On Wed, Mar 7, 2012 at 9:50 AM, Jerry McCully <for-crystallizai...@hotmail.com> wrote: >o > > Dear All; > > We purified a His tag protein by Ni-NTA and gel-filtration from > E.coli. > > We tried two endotoxin removal resins from Pierce. However, it is > hard to remove the endotoxins in the purified protein because the protein > bound to the resin as well. > > This protein contains quite a few aromatic residues and has a pI > around 6. > > Any ideas to remove the endotoxins will be highly appreciated. > > best regards, > > Jerry McCully > > -- Pius S Padayatti,PhD, Phone: 216-658-4528
