Human carbonic anhydrase II and any sulfonamide. We use a variety of
alkylsulfonamides (everyone can have a different ligand and they are
easy to model) or you can use commercially available inhibitors like
benzenesulfonamide, sulfanilamide, acetazolamide, etc. A 30 second soak
is sufficient to populate the active site. Kd values are in the
nanomolar to picomolar range for most inhibitors. 1 mM or
superstoichiometric, whichever is smaller, is sufficient.
Doug Juers at Whitman does a nice teaching lab with bovine trypsin and
benzamidine. I am gong to plagiarize that one (with Doug's permission)
for my teaching lab when I get tired of human CA-II.
Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 11/9/2012 9:12 AM, Ulrich Zander wrote:
Hi All,
For a tutorial, we would like to demonstrate the method of ligand
soaking in protein crystals. I am thinking about using some sort of
native ligand or inhibitor that can be easily identified in the
electron density rather than halide or heavy atom derivatization.
Does anybody have a suggestion for a protein/ligand combination that
could be used for that and that is commercially available?
Thanks!
Uli
