Hi all, This is a basic question and I'm sure the answer is widely known, but I'm having trouble finding it.
I'm working on my first structure. I have a dataset that I processed in XDS with a resolution cutoff of 2.35 A, although the data are extremely weak-to-nonexistent at that resolution limit. After successful molecular replacement and initial refinement, I then performed "paired refinements" against this dataset cut to various resolutions (2.95 A, 2.85 A, 2.75 A, etc). Based on the improvement in R/Rfree seen between successive pairs, it appears that the data should be cut at around 2.55 A. Here is my question: as I proceed with refinement (I'm currently using Phenix), should I now simply set "2.55 A" as the resolution limit in Phenix? Or should I go back to XDS and actually reprocess the data with the new limit (2.55 A instead of 2.35 A)? Thanks, Tom
