Hi Rohit, you should really ask Phenix questions on the Phenix bulletin board... The output means that the molecules found do not pack well in the unit cell and overlap more than a certain threshold. I would first rerun the program with the packing test switched off and look at the top solutions. If one looks alright and the overlap is just caused by some surface loop(s) in your search model that may have another position in your protein, you can just rebuild and go ahead from there. If the overlap is more serious, you can look for fewer copies or look for domains separately, if appropriate. Or increase the allowed overlap.
Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 27 Sep 2014, at 13:34, rohit kumar wrote: > Dear All, > > I am trying to solve a data of 2.9 A resolution in phenix. > But every time got this error however phenix still give a output PDB. > Can any one tell me what is the problem or any solution to overcome this > error. > > > > > $TEXT:Warning: $$ Baubles Markup $$ > ------------------------------------------------------------------------------------- > Solutions with Z-scores greater than 13.9 (the threshold indicating a > definite solution) were rejected for failing packing test > #1 TFZ=18.5 PAK=42 > #2 TFZ=18.2 PAK=38 > #3 TFZ=16.3 PAK=80 > #4 TFZ=15.6 PAK=88 > ------------------------------------------------------------------------------------- > $$ > > -- > WITH REGARDS > Rohit Kumar Singh > Lab. no. 430, > P.I. Dr. S. Gourinath, > School of Life Sciences, > Jawaharlal Nehru University > New Delhi -110067
