Dear Patrick, You may reduce the protein concentation, as well. Another option could be the *streak seeding* by exploiting the drop of your initial condition.
Good luck, V.T. On Mon, Jul 10, 2017 at 7:17 PM, Patrick Shaw Stewart <patr...@douglas.co.uk > wrote: > > Microseed them into two or three random screens. > > Search for MMS and rMMS online. > > Good luck > > Patrick > > > > > On 10 July 2017 at 15:47, Liuqing Chen <519198...@163.com> wrote: > >> hello everyone! >> I get a condition (10% w/v PEG 6000, 100mm HEPES PH7.0) in which my >> protein grow small needle like crystals, how can i optimize it to get >> bigger crystals? the attach is the crystals figure. >> thanks in advance >> sincerely >> Liuqing Chen >> > > > > -- > patr...@douglas.co.uk Douglas Instruments Ltd. > Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK > Directors: Peter Baldock, Patrick Shaw Stewart > > http://www.douglas.co.uk > Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 > <(877)%20225-2034> > Regd. England 2177994, VAT Reg. GB 480 7371 36 >
