Dear All, I am trying to refine a structure at 3.3A. Model has 60% identity to the target. Maps look OK (for 3.3A) and rebuilding in Coot is relatively straightforward. However, after some rebuilding cycles the R factors are stuck at 0.37/0.39 (REFMAC). XTRIAGE tells me that everything is normal (no twin, 98% completeness, R=3.5% in the low resolution bin), perhaps some anisotropy is present. I have already refined 2 homologous structures at resolutions going from 3.2 to 3.8 and there were no problems (final R ~ 0.21/0.24). Any advice ?
Thanks, GIA
