Dear all, We recently acquired a data set (2.0 A, P222) for a coiled coil protein (according to Itasser, QUARK, Robetta, and Phaser). Matthews coefficient indicates 1 copy of protein per ASU. Sequence of the protein is quite novel with no apparent homolog in PDB. We tried to to MR with various models (ab initio or homology based) but with little success.
We then tried to use AMPLE, but in ccp4 it always returned this error: __main__.py: error: unrecognized arguments: -use_arpwarp True (if we untick arpwarp and choose buccaneer instead, it returns -use_arpwarp False) Could anyone help? Thank you very much. Shengyang Jin Nanyang Technological University Singapore ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
