Looks like DTT adduct to me; but then again, if you did not have it, it probably is not. Petri
Petri Kursula ---------- Professor ---------- Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/rg/petrikursula petri.kurs...@uib.no ---------- Faculty of Biochemistry and Molecular Medicine Biocenter Oulu University of Oulu, Finland ---------- > On 27 Mar 2020, at 22:06, Cowan, Richard H. (Dr.) <rc...@leicester.ac.uk> > wrote: > > Hi Robbie et al., > > I'm very sure on the crystallization conditions, but I'll check the > purification for BME, although it seems unlikely, since the crystallized > protein is a Fab. > > What I would be less sure on is the age of the reagents used. Is anyone aware > of significant breakdown products which might be more reactive, particularly > the alcohols? > > Thanks, > > Dr Richard Cowan > Research Associate > <> > HWLSB 1/05 > Department of Biochemistry > University of Leicester > Lancaster Road > Leicester, LE1 <> 9HN <>, U.K. > > Phone +44 (0) 116 229 7077 > > From: robbie_joos...@hotmail.com <robbie_joos...@hotmail.com> > Sent: 27 March 2020 20:57 > To: Cowan, Richard H. (Dr.) <rc...@leicester.ac.uk> > Cc: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> > Subject: Re: [ccp4bb] Covalent Cysteine Aduct > > Are you absolutely sure about the conditions? The blob next to the sulfur > looks pretty fat so I would guess BME. > > Cheers, > Robbie > > On 27 Mar 2020 21:32, "Cowan, Richard H. (Dr.)" <rc...@leicester.ac.uk> wrote: > Hi All, > > During the current enforced shutdown, I've been going back over some older > data and spotted this in one of my structures: > > > > > It's what appears to be a covalent aduct onto a cysteine on the surface > (originally modeled as 2 waters!). The condition was optimized from the > Morpheus Screen condition D1, so MES/Imidazole pH 6.5, PEG 500MME, PEG20k and > a mix of 1,6-Hexanediol, 1-Butanol, 1,2-Propanediol, 2-Propanol, > 1,4-Butanediol and 1,3-Propanediol. The data has been cut at 1.7A, with good > stats. > > Has anyone seen anything like this before? what do you think my best bet for > modeling this is? and aduct of 1,3-propanediol? It looks too short for > 1-butanol or 1,4-butandiol, and it's linear so 2-propanol and 1,2-propanediol > seem unlikely. > > Thanks, > > Dr Richard Cowan > Research Associate > > HWLSB 1/05 > Department of Biochemistry > University of Leicester > Lancaster Road > Leicester, LE1 9HN, U.K. > > Phone +44 (0) 116 229 7077 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7Crc273%40LEICESTER.AC.UK%7Ca21cb2bb3ebe45fdcf8e08d7d2917a35%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637209394570788374&sdata=i4cJYTIxaA3NqT1u%2FscLnLR3OWxghkRLAiQ2U8zC0kI%3D&reserved=0> > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
