There are several ways to prepare “Fabs”, using the term broadly. I would dig deeper in this case to be sure a thiol adduct isn’t possible.
John > On Mar 27, 2020, at 5:38 PM, Cowan, Richard H. (Dr.) <rc...@leicester.ac.uk> > wrote: > > > Hi, > > The Fab constructs have a c-terminal cysteines on both the heavy and light > chains, which should form a disulphide. Adding reducing agent to the > purification of the protein would potentially reduce this disulphide, > possible causing issues the stability and heterogeneity? At least that's my > understanding? > > Thanks, > > Dr Richard Cowan > Research Associate > > HWLSB 1/05 > Department of Biochemistry > University of Leicester > Lancaster Road > Leicester, LE1 9HN, U.K. > > Phone +44 (0) 116 229 7077 > > From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Paul Emsley > <pems...@mrc-lmb.cam.ac.uk> > Sent: 27 March 2020 21:33 > To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> > Subject: Re: [ccp4bb] Covalent Cysteine Aduct > > > >> On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote: >> >> >> although [BME] seems unlikely, since the crystallized protein is a Fab. >> > > I don't follow. > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
