I see. Might there be some discrepancy between best practice and what actually happened? <smiley>?
On 27/03/2020 21:38, Cowan, Richard H. (Dr.) wrote:
The Fab constructs have a c-terminal cysteines on both the heavy and light chains, which should form a disulphide. Adding reducing agent to the purification of the protein would potentially reduce this disulphide, possible causing issues the stability and heterogeneity? At least that's my understanding?
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