On 22/04/2010 16:06, Peter C. wrote:

Hello again Peter R (&  everyone else at EMBOSS),

Did you manage to find out if the PCON confidence values in ABI files
are PHRED quality scores or not?

Yes ... and maybe.

The first scores are written bu the ABI basecaller.

A second set can be written by any basecaller. These may be phred quality scores but could in theory be anything.

EMBOSS will assume they are phred scores as there is no way to tell otherwise.

regards,

Peter Rice
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