I'm still not sure why you are trying to do this. If FSL did not unpack 
the volumes with the correct orientation, then you need to switch to 
mri_convert. If they are in the right orientation, then I don't 
understand what you are trying to do.
doug

On 08/16/2016 05:27 PM, Afzal, Afsana wrote:
> Hi Doug,
>
> After the coregistration failed for the majority of the subjects, I visually 
> inspected a handful of the subjects in freeview and it looks like the 
> anatomical and the functional are reversed but I could be wrong.
>
> The nifti files are unpacked from DICOMs using unpack_fsl.sh 
> (/cluster/nrg/tools/0.10.0/code/lib/shell/unpack_fsl.sh). I can point you to 
> a specific subject directory if that would be of any help.
>
> Might be worth mentioning that the subjects who failed coreg were not scanned 
> using siemens scanners but Phillips and GE scanners. Don't know if there's 
> some sort of discrepancy between the scanner coordinate frames that could 
> potentially lead to this issue.
>
> Thank you very much for your help,
>
> Afsana
> ______________________________
> Afsana Afzal
> Clinical Research Coordinator
> Massachusetts General Hospital
> Division of Neurotherapeutics
> Department of Psychiatry: Neurosciences
> 149 13th St, Room 2612
> Charlestown, MA 02129
> Phone: 617-643-5129
> Fax: 617-726-4078
>
> ________________________________________
> From: freesurfer-boun...@nmr.mgh.harvard.edu 
> [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Douglas N Greve 
> [gr...@nmr.mgh.harvard.edu]
> Sent: Tuesday, August 16, 2016 3:45 PM
> To: freesurfer@nmr.mgh.harvard.edu
> Subject: Re: [Freesurfer] Bad Coregistration - Left-Right Reversed
>
> How do you know that the structural is left-right reversed? Or that the
> fMRI is? How did you create the nifti files?
>
>
> On 08/16/2016 01:41 PM, Afzal, Afsana wrote:
>> Hi,
>>
>> I'm having trouble co-registering a bunch of subjects whose T1 is
>> left-right reversed. While the orientation of the structural file in
>> the NIfTI header is LIA and the orientation of the functional volume
>> is LAS, visual inspection shows that the structural is left-right
>> reversed. I then followed the steps outlined in
>> https://surfer.nmr.mgh.harvard.edu/fswiki/LeftRightReversal
>> <https://surfer.nmr.mgh.harvard.edu/fswiki/LeftRightReversal> to rerun
>> the reconstruction and re-registered the subject but the registration
>> mincost value is still hovering around 0.9 even with the left-right
>> reversed structural.
>>
>> I've tried running registration with SPM and FSL; neither method is
>> able to register these subjects properly.
>>
>> With --init-fsl I get the following:
>> WARNING: initial G-W contrast is negative, but expecting positive.
>> If the mov data has a T1 contrast, re-run with --T1
>>
>> and with --init-spm I get:
>> WARNING: bad orientation matrix (determinant = 0) in nifti1 file ...
>>   ... continuing.
>>
>> At this point I'm out of ideas to try out. This issue is occurring for
>> over 170 subjects (data collected outside Martinos using non-siemens
>> scanners).
>>
>> Any help would be much appreciated!
>>
>> Thanks,
>>
>> Afsana
>>
>>
>>
>> ______________________________
>> *Afsana Afzal*
>> Clinical Research Coordinator
>> Massachusetts General Hospital
>> Division of Neurotherapeutics
>> Department of Psychiatry: Neurosciences
>> 149 13th St, Room 2612
>> Charlestown, MA 02129
>> Phone: 617-643-5129
>> Fax: 617-726-4078
>>
>>
>> _______________________________________________
>> Freesurfer mailing list
>> Freesurfer@nmr.mgh.harvard.edu
>> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
> --
> Douglas N. Greve, Ph.D.
> MGH-NMR Center
> gr...@nmr.mgh.harvard.edu
> Phone Number: 617-724-2358
> Fax: 617-726-7422
>
> Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
> FileDrop: https://gate.nmr.mgh.harvard.edu/filedrop2
> www.nmr.mgh.harvard.edu/facility/filedrop/index.html
> Outgoing: ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
>
> _______________________________________________
> Freesurfer mailing list
> Freesurfer@nmr.mgh.harvard.edu
> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
>
> _______________________________________________
> Freesurfer mailing list
> Freesurfer@nmr.mgh.harvard.edu
> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
>
>

-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: https://gate.nmr.mgh.harvard.edu/filedrop2
www.nmr.mgh.harvard.edu/facility/filedrop/index.html
Outgoing: ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/

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