What command did you use to apply the transform? If mri_convert add -rt nearest 
to your command line.
The issue is that you are using trilinear interpolation our a label map instead 
of nearest neighbor

> On Apr 20, 2019, at 5:22 AM, Sadia Sheikh <sheikh.sadi...@gmail.com> wrote:
> 
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> 
> Dear FreeSurfer experts
> 
> I did brainstem segmentation using BrainstemSubstructure method. Then after 
> segmentation, I aligned all my segmented brainstem to Talairach coordinates 
> using transform=$SUBJECTS_DIR/$s/mri/transforms/talairach.xfm.
> Alignment seems to be fine. But the boundary of the aligned brainstem looks 
> distorted with different other colors. Why does the boundary look like this? 
> and how can I improve this?
> I have attached the image of brainstem after alignment.
> 
> Thanks in advance.
> 
> Regards 
> Sadia
> Research Student
> 
> 
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