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Thankyou, Sir.
It really helped me a lot.

On Sat, Apr 20, 2019 at 11:46 PM Bruce Fischl <fis...@nmr.mgh.harvard.edu>
wrote:

> What command did you use to apply the transform? If mri_convert add -rt
> nearest to your command line.
> The issue is that you are using trilinear interpolation our a label map
> instead of nearest neighbor
>
> On Apr 20, 2019, at 5:22 AM, Sadia Sheikh <sheikh.sadi...@gmail.com>
> wrote:
>
>         External Email - Use Caution
>
> Dear FreeSurfer experts
>
> I did brainstem segmentation using BrainstemSubstructure method. Then
> after segmentation, I aligned all my segmented brainstem to Talairach
> coordinates using transform=$SUBJECTS_DIR/$s/mri/transforms/talairach.xfm.
> Alignment seems to be fine. But the boundary of the aligned brainstem
> looks distorted with different other colors. Why does the boundary look
> like this? and how can I improve this?
> I have attached the image of brainstem after alignment.
>
> Thanks in advance.
>
> Regards
> Sadia
> Research Student
>
>
>
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