it looks like you are using v6. In v6, the flair might not have been normalized

On 9/23/2022 3:53 PM, Fischl, Bruce R.,PHD wrote:
I think you need to add something like -FLAIRpial or -T2pial so it uses it (in which case it will first normalize it), but hopefully someone else can confirm

cheers
Bruce
------------------------------------------------------------------------
*From:* freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of James Brown <jb1979...@gmail.com>
*Sent:* Friday, September 23, 2022 2:40 PM
*To:* freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
*Subject:* Re: [Freesurfer] White matter normalization of FLAIR images

        External Email - Use Caution

Dear Dr Bruce,
Thank you very much for your guidance.
I ran recon-all on a subject "test" using Freesurfer V6.0 and the following command line is reported in the "recon-all.log":

/usr/local/freesurfer/6.0/bin/recon-all -all -qcache -i T1.nii.gz -flair FLAIR.nii.gz -s test

When I search for normalization of flair, I only find how Freesurfer normalized T1. I searched for "nonmax_suppress" and it is not in the log file. I am just wondering what I am doing wrong. Thanks again for any highlights about the correct method to normalize FLAIR

Sincerely,



Hi JamesB

there is T2/FLAIR normalization in recon-all if you process it through there. You can look for "nonmax_suppress" if you want to find the command line

cheers
Bruce

On Fri, Sep 23, 2022 at 2:06 PM James Brown <jb1979...@gmail.com> wrote:

    Dear Freesurfer experts,
    We appreciate any advice or guidance from your community of
    professionals and experts in the field about the issue below.

    Thank you for your kindness.

    Dear Freesurfer experts,
    In order to normalize white matter intensity of T1 images and
    correct for bias field. We do the following steps:
    mri_convert T1.nii.gz T1.mgz
    mri_nu_correct.mni --i T1.mgz --o T1_N3.mgz --n 2
    mri_normalize -g 1 T1_N3.mgzT1_norm.mgz
    mri_convert T1_norm.mgz T1_norm.nii.gz

    We would like to apply bias field correction on FLAIR images then
    normalize white matter intensity.
    We tried N4biasfieldcorrection from ANTS but it didn't work as
    expected. Reviewing documents about N4biasfieldcorrection we
    realize that the algorithm has been developed to correct bias
    field correction in T1 not clear if it works on FLAIR images.

    Are the steps above valid for normalizing FLAIR images or can it
    only be applied on T1 images? I would like to send a
    "recon-all.log" file that includes the processing steps of FLAIR.
    Is there any link I can use to upload the file. It gets rejected
    every time I send it due to size limitations.

    We appreciate any feedback about the proper way to correct FLAIR
    images for bias fields and normalize white matter intensity in
    FLAIR images.

    Thank you
    JamesB

    On Wed, Sep 21, 2022 at 10:18 AM James Brown <jb1979...@gmail.com>
    wrote:

        Dear Freesurfer experts,
        In order to normalize white matter intensity of T1 images and
        correct for bias field. We do the following steps:
        mri_convert T1.nii.gz T1.mgz
        mri_nu_correct.mni --i T1.mgz --o T1_N3.mgz --n 2
        mri_normalize -g 1 T1_N3.mgzT1_norm.mgz
        mri_convert T1_norm.mgz T1_norm.nii.gz

        We would like to apply bias field correction on FLAIR images
        then normalize white matter intensity.
        We tried N4biasfieldcorrection from ANTS but it didn't work as
        expected. Reviewing documents about N4biasfieldcorrection we
        realize that the algorithm has been developed to correct bias
        field correction in T1 not clear if it works on FLAIR images.

        Are the steps above valid for normalizing FLAIR images or can
        it only be applied on T1 images? I would like to send a
        "recon-all.log" file that includes the processing steps of
        FLAIR. Is there any link I can use to upload the file. It gets
        rejected every time I send it due to size limitations.

        We appreciate any feedback about the proper way to correct
        FLAIR images for bias fields and normalize white matter
        intensity in FLAIR images.

        Thank you
        JamesB


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