External Email - Use Caution Hi Yujing,
Sure. the results of applying mri_info are attached below Bests, Yazdan. On Fri, 6 Oct 2023 at 23:19, Huang, Yujing <yhuan...@mgh.harvard.edu> wrote: > External Email - Use Caution > > Hi Yazdan, > > > > Thank you for the detailed explanation. > > > > I’m wondering if you could send output of mri_info for Image.nii, > fsl_resampled.nii.gz, fslregistered.nii.gz, freesurfer.nii, and output from > ANTs. > > > > Best, > > > > Yujing > > > > *From:* freesurfer-boun...@nmr.mgh.harvard.edu < > freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Yazdan Rezaee > Jouryabi > *Sent:* Friday, September 29, 2023 12:19 PM > *To:* Freesurfer support list <freesurfer@nmr.mgh.harvard.edu> > *Subject:* Re: [Freesurfer] lta_convert issue with ants Registration > Matrix > > > > * External Email - Use Caution * > > Hi Yujing, > > I'll try to explain it more explicitly, I rerun my Code and show the > results beyond attaching the Registration files too. > > > > Consider a 3D-Nifti image called "Image.nii" the steps bellow executed in > order: > > 0- Every time we Will generate a random Registration Matrix and Save it > under the name "fsl.mat" which is the desired Registration matrix. > > > ============================================================================== > > fsl.mat := > > [[ 0.95509431, -0.28438761, 0.08317783, -5. ] > > [ 0.2961434, 0.90701597, -0.29936787, -5. ] > > [ 0.00969289, 0.31055712, 0.9505053, -2. ] > > [ 0., 0., 0., 1. ]] > > > ============================================================================== > > > > 1- using a known registration matrice (called and attached in the first > message and this message as "fsl.mat") resample the "Image.nii" to second > Image called "fsl_resampled.nii" > > Command : flirt -ref ./Image.nii -in ./Image.nii -applyxfm -init > ./fsl.mat -out ./fsl_resampled.nii -interp spline > > 2- find out the registration matrices using Registration Methods, here we > will use FSL, FreesSurefer, ANTs: > > 2.1. FSL Commands: > > > =============================================================================== > > Command : flirt -in ./Image.nii -out ./fslregistered.nii.gz -ref > ./fsl_resampled.nii.gz -cost normmi -dof 6 -omat ./fslregistered.mat > -interp spline > > > =============================================================================== > > fslregistered : > > 0.9551458402 -0.2842262621 0.08313877187 -5.029802253 > > 0.2959789517 0.9070999982 -0.2992758021 -5.014360215 > > 0.009646861275 0.3104593324 0.9505376886 -1.971170444 > > 0 0 0 1 > ======================================================= > > The Registration Matrice in this Case Called "fslregistered.mat", using > lta_convert we will make "fslregistered.lta" > > Command : lta_convert --infsl ./fsl/fslregistered.mat --outlta > ./fsl/fslregistered.lta --src ./Image.nii --trg ./fsl/fslregistered.nii.gz > --ltavox2vox > > > =============================================================================== > > fslregistered.lta : > > 9.551459550857544e-01 -2.368552237749100e-01 6.928230822086334e-02 > -4.191513061523438e+00 > > 3.551747798919678e-01 9.071001410484314e-01 -2.992758452892303e-01 > -5.014381408691406e+00 > > 1.157622877508402e-02 3.104593753814697e-01 9.505378007888794e-01 > -1.971183776855469e+00 > > 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 > 1.000000000000000e+00 > > 2.2. FreeSurfer Commands: > > > =============================================================================== > > Command : mri_robust_register --mov ./Image.nii --dst > ./fsl_resampled.nii.gz --mapmov ./freesurfer.nii --lta freesurfer.lta > --halfmov half-mov.nii --halfdst halfdst.nii --halfmovlta h1.lta > --halfdstlta h2.lta --iscale --satit > > =============================================================================== > freesurfer.lta : > type = 0 # LINEAR_VOX_TO_VOX > > nxforms = 1 > > mean = 87.0000 129.0000 106.0000 > > sigma = 10000.0000 > > 1 4 4 > > 9.550938606262207e-01 -2.369909435510635e-01 6.931482255458832e-02 > -4.167178630828857e+00 > > 3.553738296031952e-01 9.070160388946533e-01 -2.993661761283875e-01 > -4.999662876129150e+00 > > 1.163147203624249e-02 3.105554580688477e-01 9.505058526992798e-01 > -2.000635147094727e+00 > > 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 > 1.000000000000000e+00 > > > ================================================================================ > > The Registration Matrice in this Case Called "freesurfer.lta", using > lta_convert we will do the conversion and build the FSL-Space > "freesurfer.mat" > > Command : lta_convert --inlta ./freesurfer/freesurfer.lta --outfsl > ./freesurfer/freesurfer.mat --src ./Image.nii --trg ./fsl_resampled.nii.gz > > ======================================================== > freesurfer.mat : > 0.95509410 -0.28438917 0.08317780 -5.00063801 > > 0.29614490 0.90701610 -0.29936624 -4.99965954 > > 0.00969290 0.31055552 0.95050597 -2.00067019 > > 0.00000000 0.00000000 0.00000000 1.00000000 > > > ================================================================================ > > 2.3. ANTs Commands: > > > ================================================================================ > > Commands : antsRegistrationSyNQuick.sh -d 3 -f ./fsl_resampled.nii.gz > -m ./Image.nii -o ants_ -t r > > Using the ConvertTransform File we will make the "ants.txt" > > Command : ConvertTransformFile 3 ./ANTS/ants_0GenericAffine.mat > ./ANTS/ants.txt > > > ================================================================================ > > ants.txt: > > #Insight Transform File V1.0 > > #Transform 0 > > Transform: AffineTransform_double_3_3 > > Parameters: 0.9996357319973235 -0.01682948721742278 -0.021099091785527296 > 0.015864886388920783 0.9988573896699559 -0.045080156184165145 > 0.02183365965755943 0.04472900023162478 0.9987605357863502 -2.215029749904149 > 1.2122977228542815 -6.0667455324006445 > > FixedParameters: 5.195914900051355 -10.250280974097581 -6.670270481513606 > > > ================================================================================ > > Using lta_convert we first make "ants.lta" > > Command: lta_convert --initk ./ANTS/ants.txt --outlta ./ANTS/ants.lta > --src ./Image.nii --trg ./fsl_resampled.nii.gz --ltavox2vox > > > ================================================================================ > > type = 0 # LINEAR_VOX_TO_VOX > > nxforms = 1 > > mean = 0.0000 0.0000 0.0000 > > sigma = 1.0000 > > 1 4 4 > > 9.996357560157776e-01 -1.322073861956596e-02 1.819471642374992e-02 > 1.924201965332031e+00 > > 2.019538544118404e-02 9.988574385643005e-01 -4.472899809479713e-02 > 3.623344421386719e+00 > > -2.531890943646431e-02 4.508015885949135e-02 9.987605214118958e-01 > 2.642463684082031e+00 > > 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 > 1.000000000000000e+00 > ================================================================================= > Using the lta_convert we make the "ants.mat", the FSL-Spaced Registration > matrix for ANTs > Command: lta_convert --inlta ./ANTS/ants.lta --outfsl ./ANTS/ants.mat > --src ./Image.nii --trg ./fsl_resampled.nii.gz > ================================================================================= > 0.99963582 -0.01586489 0.02183366 2.30903101 > > 0.01682949 0.99885744 -0.04472899 3.62335658 > > -0.02109909 0.04508016 0.99876052 2.64244890 > > 0.00000000 0.00000000 0.00000000 1.00000000 > > > As we could see there are problems in 'ants.lta' and 'ants.mat', I'm not sure > Yet but I Suspect that the "PSR" Orientation of the Image Which is a > Left-handed coordination System may cause that because in Another Experiment > with "LAS" these problem had been occured but in another "RAS" Image the > lta_convertor worked precisely with no problem. > > The Registration Files are attached for More Information about Image's > Geometry Sform's and Other ... > > > Best, > > Yazdan > > > > > > > > On Mon, 25 Sept 2023 at 17:16, Huang, Yujing <yhuan...@mgh.harvard.edu> > wrote: > > * External Email - Use Caution * > > Hi Yazdan, > > > > I’m trying to understand the issues. > > > > Can you specify the commands used to generate the matrices attached to > your email? And give more details of the issues you are seeing? > > > > Best, > > > > Yujing > > > > *From:* freesurfer-boun...@nmr.mgh.harvard.edu < > freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Yazdan Rezaee > Jouryabi > *Sent:* Monday, September 25, 2023 1:49 AM > *To:* freesurfer@nmr.mgh.harvard.edu > *Subject:* [Freesurfer] lta_convert issue with ants Registration Matrix > > > > * External Email - Use Caution * > > Dear all, Hi > > > > for checking registration i used fsl applyxfm on an Image(1)(Called > Image.nii ) with specific Registration Matrice (Which we suppose to receive > when we apply registration Methods) and Generate the "fsl_resampled.nii.gz" > (Note: Image Orientations were LAS and in one other Experiment with also > the same problem PSR) > > > > for our case i will attach the "fsl.mat" (Desired Matrix) > > > > using, fsl, freesurfer, and ants i got the correct results (let's call > them "fslregistered.mat" and "freesurfer.mat" the converted lta to > fsl-liked Matrix) Everything is OK till now > > > > But the converted lta-like and fsl-like matrix for ANTs (Which are called > ants.lta, and ants.mat) using the lines below: > > - ConvertTransformFile 3 ./ANTS/ants_0GenericAffine.mat ./ANTS/ants.txt > > - lta_convert --initk ./ANTS/ants.txt --outlta ./ANTS/ants.lta --src > ./Image.nii --trg ./fsl_resampled.nii.gz --ltavox2vox > > - lta_convert --inlta ./ANTS/ants.lta --outfsl ./ANTS/ants.mat --src > ./Image.nii --trg ./fsl_resampled.nii.gz > > > > As All Results Images (Registered Image of all Methods) are aligned > perfectly i think that there might be some issues with lta_convert > > > > > > Best Regards > > Yazdan Rezaee J > > > > > > > *P.S. *" I've checked the one similar mail on this topic and the > conclusion on that was about (sform, qform) incompatibility but the affine > matrices of S-form and q-form are equivalent in my case." > > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > *MailScanner has detected a possible fraud attempt from > "secure-web.cisco.com" claiming to be* > https://secure-web.cisco.com/1BStSAxLh5BeN2L3rU2frK5cGS07arbjFgCFDCXjer8mI9E6QhC8axsTzJmtNHA0f1DD52gOLn7V-0bXwHcjGpMgOvj5Ej9o0ngUReq4erjdQzvYap5kCRZ7e1R0AlzG3ItDCyzjDBFs1Hwuf_QGSCA0SbXehYcVoataMjv341ofR-qoXsG8G2K8Q2d1z1G3CluiFixSCjME4890bumFvO47QQG0nNCEDOPpFNuPHYPbFtpXrvY-tFm2uqnoNPMEp5d-TAX_caWLaTC52xtF8vU_f4PLeM-ImIm6-4ZiPLVGGJ6bU0ZNKXVhnhIG9oCdTp1_UXPx1xKVwGkz_KsQhyQ/https%3A%2F%2Fmail.nmr.mgh.harvard.edu%2Fmailman%2Flistinfo%2Ffreesurfer > <https://secure-web.cisco.com/1CVysUaKA0QPJhHH2wwhYBipzUhFEaeL8kkwOtuATULgzm-ARVtRifTqrnuMch9mPrhoX5WsrgJCZI4n6LV0QrBOiwz8JOJSZcKXl0-srjhHE7Ez1eAdpgDrWIqslv1yZuu9lDDu4UrcCR_1k8-OHZM1lX5im7TD25ziCO5ibM6spWh-yZJ_pdgSrOWg3COhe2JLUiyCL3VPWaa-l3gJ-VY8E3fKgFi47mdINsuywYT2Z2bpFzMA0qVMOcxBZP6V2Jz-SpyfFbeS55S0r_f_lNm2JigMyuajjviAgHw-npf_6HYFs541IzQp4hzn0kOTN/https%3A%2F%2Fmail.nmr.mgh.harvard.edu%2Fmailman%2Flistinfo%2Ffreesurfer> > The information in this e-mail is intended only for the person to whom it > is addressed. 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Volume information for Image.nii type: nii dimensions: 170 x 256 x 256 voxel sizes: 1.200000, 1.000000, 1.000000 type: SHORT (4) fov: 204.000 dof: 1 xstart: -102.0, xend: 102.0 ystart: -128.0, yend: 128.0 zstart: -128.0, zend: 128.0 TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 degrees nframes: 1 PhEncDir: UNKNOWN FieldStrength: 0.000000 ras xform present xform info: x_r = -1.0000, y_r = -0.0000, z_r = -0.0000, c_r = -0.6003 : x_a = 0.0000, y_a = 1.0000, z_a = 0.0000, c_a = 17.5182 : x_s = -0.0000, y_s = -0.0000, z_s = 1.0000, c_s = 16.7755 Orientation : LAS Primary Slice Direction: axial voxel to ras transform: -1.2000 -0.0000 -0.0000 101.3997 0.0000 1.0000 0.0000 -110.4818 -0.0000 -0.0000 1.0000 -111.2245 0.0000 0.0000 0.0000 1.0000 voxel-to-ras determinant -1.2 ras to voxel transform: -0.8333 -0.0000 -0.0000 84.4998 -0.0000 1.0000 -0.0000 110.4818 -0.0000 -0.0000 1.0000 111.2245 -0.0000 -0.0000 -0.0000 1.0000
Volume information for fsl_resampled.nii.gz type: nii dimensions: 170 x 256 x 256 voxel sizes: 1.200000, 1.000000, 1.000000 type: SHORT (4) fov: 204.000 dof: 1 xstart: -102.0, xend: 102.0 ystart: -128.0, yend: 128.0 zstart: -128.0, zend: 128.0 TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 degrees nframes: 1 PhEncDir: UNKNOWN FieldStrength: 0.000000 ras xform present xform info: x_r = -1.0000, y_r = -0.0000, z_r = -0.0000, c_r = -0.6003 : x_a = 0.0000, y_a = 1.0000, z_a = 0.0000, c_a = 17.5182 : x_s = -0.0000, y_s = -0.0000, z_s = 1.0000, c_s = 16.7755 Orientation : LAS Primary Slice Direction: axial voxel to ras transform: -1.2000 -0.0000 -0.0000 101.3997 0.0000 1.0000 0.0000 -110.4818 -0.0000 -0.0000 1.0000 -111.2245 0.0000 0.0000 0.0000 1.0000 voxel-to-ras determinant -1.2 ras to voxel transform: -0.8333 -0.0000 -0.0000 84.4998 -0.0000 1.0000 -0.0000 110.4818 -0.0000 -0.0000 1.0000 111.2245 -0.0000 -0.0000 -0.0000 1.0000
Volume information for fslregistered.nii.gz type: nii dimensions: 170 x 256 x 256 voxel sizes: 1.200000, 1.000000, 1.000000 type: SHORT (4) fov: 204.000 dof: 1 xstart: -102.0, xend: 102.0 ystart: -128.0, yend: 128.0 zstart: -128.0, zend: 128.0 TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 degrees nframes: 1 PhEncDir: UNKNOWN FieldStrength: 0.000000 ras xform present xform info: x_r = -1.0000, y_r = -0.0000, z_r = -0.0000, c_r = -0.6003 : x_a = 0.0000, y_a = 1.0000, z_a = 0.0000, c_a = 17.5182 : x_s = -0.0000, y_s = -0.0000, z_s = 1.0000, c_s = 16.7755 Orientation : LAS Primary Slice Direction: axial voxel to ras transform: -1.2000 -0.0000 -0.0000 101.3997 0.0000 1.0000 0.0000 -110.4818 -0.0000 -0.0000 1.0000 -111.2245 0.0000 0.0000 0.0000 1.0000 voxel-to-ras determinant -1.2 ras to voxel transform: -0.8333 -0.0000 -0.0000 84.4998 -0.0000 1.0000 -0.0000 110.4818 -0.0000 -0.0000 1.0000 111.2245 -0.0000 -0.0000 -0.0000 1.0000
Volume information for freesurfer.nii type: nii dimensions: 170 x 256 x 256 voxel sizes: 1.200000, 1.000000, 1.000000 type: SHORT (4) fov: 204.000 dof: 1 xstart: -102.0, xend: 102.0 ystart: -128.0, yend: 128.0 zstart: -128.0, zend: 128.0 TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 degrees nframes: 1 PhEncDir: UNKNOWN FieldStrength: 0.000000 ras xform present xform info: x_r = -1.0000, y_r = -0.0000, z_r = -0.0000, c_r = -0.6003 : x_a = 0.0000, y_a = 1.0000, z_a = 0.0000, c_a = 17.5182 : x_s = -0.0000, y_s = -0.0000, z_s = 1.0000, c_s = 16.7755 Orientation : LAS Primary Slice Direction: axial voxel to ras transform: -1.2000 -0.0000 -0.0000 101.3997 0.0000 1.0000 0.0000 -110.4818 -0.0000 -0.0000 1.0000 -111.2245 0.0000 0.0000 0.0000 1.0000 voxel-to-ras determinant -1.2 ras to voxel transform: -0.8333 -0.0000 -0.0000 84.4998 -0.0000 1.0000 -0.0000 110.4818 -0.0000 -0.0000 1.0000 111.2245 -0.0000 -0.0000 -0.0000 1.0000
niiRead(): detected input as 64 bit double, reading in as 32 bit float Volume information for ants_Warped.nii.gz type: nii dimensions: 170 x 256 x 256 voxel sizes: 1.200000, 1.000000, 1.000000 type: FLOAT (3) fov: 204.000 dof: 1 xstart: -102.0, xend: 102.0 ystart: -128.0, yend: 128.0 zstart: -128.0, zend: 128.0 TR: 0.00 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 degrees nframes: 1 PhEncDir: UNKNOWN FieldStrength: 0.000000 ras xform present xform info: x_r = -1.0000, y_r = -0.0000, z_r = 0.0000, c_r = -0.6003 : x_a = -0.0000, y_a = 1.0000, z_a = -0.0000, c_a = 17.5182 : x_s = 0.0000, y_s = 0.0000, z_s = 1.0000, c_s = 16.7755 Orientation : LAS Primary Slice Direction: axial voxel to ras transform: -1.2000 -0.0000 0.0000 101.3997 -0.0000 1.0000 -0.0000 -110.4818 0.0000 0.0000 1.0000 -111.2245 0.0000 0.0000 0.0000 1.0000 voxel-to-ras determinant -1.2 ras to voxel transform: -0.8333 -0.0000 -0.0000 84.4998 -0.0000 1.0000 -0.0000 110.4818 -0.0000 -0.0000 1.0000 111.2245 -0.0000 -0.0000 -0.0000 1.0000
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