External Email - Use Caution        

Hi Yujing,

Sure.

the results of applying mri_info are attached below

Bests,
Yazdan.

On Fri, 6 Oct 2023 at 23:19, Huang, Yujing <yhuan...@mgh.harvard.edu> wrote:

>         External Email - Use Caution
>
> Hi Yazdan,
>
>
>
> Thank you for the detailed explanation.
>
>
>
> I’m wondering if you could send output of mri_info for Image.nii,
> fsl_resampled.nii.gz, fslregistered.nii.gz, freesurfer.nii, and output from
> ANTs.
>
>
>
> Best,
>
>
>
> Yujing
>
>
>
> *From:* freesurfer-boun...@nmr.mgh.harvard.edu <
> freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Yazdan Rezaee
> Jouryabi
> *Sent:* Friday, September 29, 2023 12:19 PM
> *To:* Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
> *Subject:* Re: [Freesurfer] lta_convert issue with ants Registration
> Matrix
>
>
>
> *        External Email - Use Caution        *
>
> Hi Yujing,
>
> I'll try to explain it more explicitly, I rerun my Code and show the
> results beyond attaching the Registration files too.
>
>
>
> Consider a 3D-Nifti image called "Image.nii" the steps bellow executed in
> order:
>
> 0- Every time we Will generate a random Registration Matrix and Save it
> under the name "fsl.mat" which is the desired Registration matrix.
>
>
> ==============================================================================
>
> fsl.mat :=
>
> [[ 0.95509431, -0.28438761, 0.08317783, -5.        ]
>
>  [ 0.2961434,   0.90701597, -0.29936787, -5.        ]
>
>  [ 0.00969289,  0.31055712,  0.9505053,  -2.        ]
>
>  [ 0.,          0.,          0.,          1.        ]]
>
>
> ==============================================================================
>
>
>
> 1- using a known registration matrice (called and attached in the first
> message and this message as "fsl.mat") resample the "Image.nii" to second
> Image called "fsl_resampled.nii"
>
>       Command :   flirt -ref ./Image.nii -in ./Image.nii -applyxfm -init
> ./fsl.mat -out ./fsl_resampled.nii -interp spline
>
> 2- find out the registration matrices using Registration Methods, here we
> will use FSL, FreesSurefer, ANTs:
>
> 2.1. FSL Commands:
>
>
> ===============================================================================
>
>       Command : flirt -in ./Image.nii -out ./fslregistered.nii.gz -ref
> ./fsl_resampled.nii.gz -cost normmi -dof 6 -omat ./fslregistered.mat
> -interp spline
>
>
> ===============================================================================
>
> fslregistered :
>
> 0.9551458402  -0.2842262621  0.08313877187  -5.029802253
>
> 0.2959789517  0.9070999982  -0.2992758021  -5.014360215
>
> 0.009646861275  0.3104593324  0.9505376886  -1.971170444
>
> 0  0  0  1
> =======================================================
>
> The Registration Matrice in this Case Called "fslregistered.mat", using
> lta_convert we will make "fslregistered.lta"
>
>       Command : lta_convert --infsl ./fsl/fslregistered.mat --outlta
> ./fsl/fslregistered.lta --src ./Image.nii --trg ./fsl/fslregistered.nii.gz
> --ltavox2vox
>
>
> ===============================================================================
>
> fslregistered.lta :
>
> 9.551459550857544e-01 -2.368552237749100e-01 6.928230822086334e-02 
> -4.191513061523438e+00
>
> 3.551747798919678e-01 9.071001410484314e-01 -2.992758452892303e-01 
> -5.014381408691406e+00
>
> 1.157622877508402e-02 3.104593753814697e-01 9.505378007888794e-01 
> -1.971183776855469e+00
>
> 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 
> 1.000000000000000e+00
>
> 2.2. FreeSurfer Commands:
>
>
> ===============================================================================
>
>       Command : mri_robust_register --mov ./Image.nii --dst
> ./fsl_resampled.nii.gz --mapmov ./freesurfer.nii --lta freesurfer.lta
> --halfmov half-mov.nii --halfdst halfdst.nii --halfmovlta h1.lta
> --halfdstlta h2.lta --iscale --satit
>
> ===============================================================================
> freesurfer.lta :
> type      = 0 # LINEAR_VOX_TO_VOX
>
> nxforms   = 1
>
> mean      = 87.0000 129.0000 106.0000
>
> sigma     = 10000.0000
>
> 1 4 4
>
> 9.550938606262207e-01 -2.369909435510635e-01 6.931482255458832e-02 
> -4.167178630828857e+00
>
> 3.553738296031952e-01 9.070160388946533e-01 -2.993661761283875e-01 
> -4.999662876129150e+00
>
> 1.163147203624249e-02 3.105554580688477e-01 9.505058526992798e-01 
> -2.000635147094727e+00
>
> 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 
> 1.000000000000000e+00
>
>
> ================================================================================
>
> The Registration Matrice in this Case Called "freesurfer.lta", using
> lta_convert we will do the conversion and build the FSL-Space
> "freesurfer.mat"
>
>       Command : lta_convert --inlta ./freesurfer/freesurfer.lta --outfsl
> ./freesurfer/freesurfer.mat --src ./Image.nii --trg ./fsl_resampled.nii.gz
>
> ========================================================
> freesurfer.mat :
>    0.95509410   -0.28438917    0.08317780   -5.00063801
>
>    0.29614490    0.90701610   -0.29936624   -4.99965954
>
>    0.00969290    0.31055552    0.95050597   -2.00067019
>
>    0.00000000    0.00000000    0.00000000    1.00000000
>
>
> ================================================================================
>
> 2.3. ANTs Commands:
>
>
> ================================================================================
>
>      Commands : antsRegistrationSyNQuick.sh -d 3 -f ./fsl_resampled.nii.gz
> -m ./Image.nii -o ants_ -t r
>
>  Using the ConvertTransform File we will make the "ants.txt"
>
>      Command : ConvertTransformFile 3 ./ANTS/ants_0GenericAffine.mat
> ./ANTS/ants.txt
>
>
> ================================================================================
>
> ants.txt:
>
> #Insight Transform File V1.0
>
> #Transform 0
>
> Transform: AffineTransform_double_3_3
>
> Parameters: 0.9996357319973235 -0.01682948721742278 -0.021099091785527296 
> 0.015864886388920783 0.9988573896699559 -0.045080156184165145 
> 0.02183365965755943 0.04472900023162478 0.9987605357863502 -2.215029749904149 
> 1.2122977228542815 -6.0667455324006445
>
> FixedParameters: 5.195914900051355 -10.250280974097581 -6.670270481513606
>
>
> ================================================================================
>
> Using lta_convert we first make "ants.lta"
>
>      Command: lta_convert --initk ./ANTS/ants.txt --outlta ./ANTS/ants.lta
> --src ./Image.nii --trg ./fsl_resampled.nii.gz --ltavox2vox
>
>
> ================================================================================
>
> type      = 0 # LINEAR_VOX_TO_VOX
>
> nxforms   = 1
>
> mean      = 0.0000 0.0000 0.0000
>
> sigma     = 1.0000
>
> 1 4 4
>
> 9.996357560157776e-01 -1.322073861956596e-02 1.819471642374992e-02 
> 1.924201965332031e+00
>
> 2.019538544118404e-02 9.988574385643005e-01 -4.472899809479713e-02 
> 3.623344421386719e+00
>
> -2.531890943646431e-02 4.508015885949135e-02 9.987605214118958e-01 
> 2.642463684082031e+00
>
> 0.000000000000000e+00 0.000000000000000e+00 0.000000000000000e+00 
> 1.000000000000000e+00
> =================================================================================
> Using the lta_convert we make the "ants.mat", the FSL-Spaced Registration 
> matrix for ANTs
>       Command: lta_convert --inlta ./ANTS/ants.lta --outfsl ./ANTS/ants.mat 
> --src ./Image.nii --trg ./fsl_resampled.nii.gz
> =================================================================================
>   0.99963582   -0.01586489    0.02183366    2.30903101
>
>    0.01682949    0.99885744   -0.04472899    3.62335658
>
>   -0.02109909    0.04508016    0.99876052    2.64244890
>
>    0.00000000    0.00000000    0.00000000    1.00000000
>
>
> As we could see there are problems in 'ants.lta' and 'ants.mat', I'm not sure 
> Yet but I Suspect that the "PSR" Orientation of the Image Which is a 
> Left-handed coordination System may cause that because in Another Experiment 
> with "LAS" these problem had been occured but in another "RAS" Image the 
> lta_convertor worked precisely with no problem.
>
> The Registration Files are attached for More Information about Image's 
> Geometry Sform's and Other ...
>
>
> Best,
>
> Yazdan
>
>
>
>
>
>
>
> On Mon, 25 Sept 2023 at 17:16, Huang, Yujing <yhuan...@mgh.harvard.edu>
> wrote:
>
> *        External Email - Use Caution        *
>
> Hi Yazdan,
>
>
>
> I’m trying to understand the issues.
>
>
>
> Can you specify the commands used to generate the matrices attached to
> your email? And give more details of the issues you are seeing?
>
>
>
> Best,
>
>
>
> Yujing
>
>
>
> *From:* freesurfer-boun...@nmr.mgh.harvard.edu <
> freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Yazdan Rezaee
> Jouryabi
> *Sent:* Monday, September 25, 2023 1:49 AM
> *To:* freesurfer@nmr.mgh.harvard.edu
> *Subject:* [Freesurfer] lta_convert issue with ants Registration Matrix
>
>
>
> *        External Email - Use Caution        *
>
> Dear all, Hi
>
>
>
> for checking registration i used fsl applyxfm on an Image(1)(Called
> Image.nii ) with specific Registration Matrice (Which we suppose to receive
> when we apply registration Methods) and Generate the "fsl_resampled.nii.gz"
> (Note: Image Orientations were LAS and in one other Experiment with also
> the same problem PSR)
>
>
>
> for our case i will attach the "fsl.mat" (Desired Matrix)
>
>
>
> using, fsl, freesurfer, and ants i got the correct results (let's call
> them "fslregistered.mat" and "freesurfer.mat" the converted lta to
> fsl-liked Matrix) Everything is OK till now
>
>
>
>  But the converted lta-like and fsl-like matrix for ANTs (Which are called
> ants.lta, and ants.mat) using the lines below:
>
> - ConvertTransformFile 3 ./ANTS/ants_0GenericAffine.mat ./ANTS/ants.txt
>
> - lta_convert --initk ./ANTS/ants.txt --outlta ./ANTS/ants.lta --src
> ./Image.nii --trg ./fsl_resampled.nii.gz --ltavox2vox
>
> - lta_convert --inlta ./ANTS/ants.lta --outfsl ./ANTS/ants.mat --src
> ./Image.nii --trg ./fsl_resampled.nii.gz
>
>
>
> As All Results Images (Registered Image of all Methods) are aligned
> perfectly i think that there might be some issues with lta_convert
>
>
>
>
>
> Best Regards
>
> Yazdan Rezaee J
>
>
>
>
>
>
> *P.S. *" I've checked the one similar mail on this topic and the
> conclusion on that was about (sform, qform) incompatibility but the affine
> matrices of S-form and q-form are equivalent in my case."
>
>
>
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Volume information for Image.nii
          type: nii
    dimensions: 170 x 256 x 256
   voxel sizes: 1.200000, 1.000000, 1.000000
          type: SHORT (4)
           fov: 204.000
           dof: 1
        xstart: -102.0, xend: 102.0
        ystart: -128.0, yend: 128.0
        zstart: -128.0, zend: 128.0
            TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 
degrees
       nframes: 1
       PhEncDir: UNKNOWN
       FieldStrength: 0.000000
ras xform present
    xform info: x_r =  -1.0000, y_r =  -0.0000, z_r =  -0.0000, c_r =    -0.6003
              : x_a =   0.0000, y_a =   1.0000, z_a =   0.0000, c_a =    17.5182
              : x_s =  -0.0000, y_s =  -0.0000, z_s =   1.0000, c_s =    16.7755
Orientation   : LAS
Primary Slice Direction: axial

voxel to ras transform:
               -1.2000  -0.0000  -0.0000   101.3997
                0.0000   1.0000   0.0000  -110.4818
               -0.0000  -0.0000   1.0000  -111.2245
                0.0000   0.0000   0.0000     1.0000

voxel-to-ras determinant -1.2

ras to voxel transform:
               -0.8333  -0.0000  -0.0000    84.4998
               -0.0000   1.0000  -0.0000   110.4818
               -0.0000  -0.0000   1.0000   111.2245
               -0.0000  -0.0000  -0.0000     1.0000
Volume information for fsl_resampled.nii.gz
          type: nii
    dimensions: 170 x 256 x 256
   voxel sizes: 1.200000, 1.000000, 1.000000
          type: SHORT (4)
           fov: 204.000
           dof: 1
        xstart: -102.0, xend: 102.0
        ystart: -128.0, yend: 128.0
        zstart: -128.0, zend: 128.0
            TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 
degrees
       nframes: 1
       PhEncDir: UNKNOWN
       FieldStrength: 0.000000
ras xform present
    xform info: x_r =  -1.0000, y_r =  -0.0000, z_r =  -0.0000, c_r =    -0.6003
              : x_a =   0.0000, y_a =   1.0000, z_a =   0.0000, c_a =    17.5182
              : x_s =  -0.0000, y_s =  -0.0000, z_s =   1.0000, c_s =    16.7755
Orientation   : LAS
Primary Slice Direction: axial

voxel to ras transform:
               -1.2000  -0.0000  -0.0000   101.3997
                0.0000   1.0000   0.0000  -110.4818
               -0.0000  -0.0000   1.0000  -111.2245
                0.0000   0.0000   0.0000     1.0000

voxel-to-ras determinant -1.2

ras to voxel transform:
               -0.8333  -0.0000  -0.0000    84.4998
               -0.0000   1.0000  -0.0000   110.4818
               -0.0000  -0.0000   1.0000   111.2245
               -0.0000  -0.0000  -0.0000     1.0000
Volume information for fslregistered.nii.gz
          type: nii
    dimensions: 170 x 256 x 256
   voxel sizes: 1.200000, 1.000000, 1.000000
          type: SHORT (4)
           fov: 204.000
           dof: 1
        xstart: -102.0, xend: 102.0
        ystart: -128.0, yend: 128.0
        zstart: -128.0, zend: 128.0
            TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 
degrees
       nframes: 1
       PhEncDir: UNKNOWN
       FieldStrength: 0.000000
ras xform present
    xform info: x_r =  -1.0000, y_r =  -0.0000, z_r =  -0.0000, c_r =    -0.6003
              : x_a =   0.0000, y_a =   1.0000, z_a =   0.0000, c_a =    17.5182
              : x_s =  -0.0000, y_s =  -0.0000, z_s =   1.0000, c_s =    16.7755
Orientation   : LAS
Primary Slice Direction: axial

voxel to ras transform:
               -1.2000  -0.0000  -0.0000   101.3997
                0.0000   1.0000   0.0000  -110.4818
               -0.0000  -0.0000   1.0000  -111.2245
                0.0000   0.0000   0.0000     1.0000

voxel-to-ras determinant -1.2

ras to voxel transform:
               -0.8333  -0.0000  -0.0000    84.4998
               -0.0000   1.0000  -0.0000   110.4818
               -0.0000  -0.0000   1.0000   111.2245
               -0.0000  -0.0000  -0.0000     1.0000
Volume information for freesurfer.nii
          type: nii
    dimensions: 170 x 256 x 256
   voxel sizes: 1.200000, 1.000000, 1.000000
          type: SHORT (4)
           fov: 204.000
           dof: 1
        xstart: -102.0, xend: 102.0
        ystart: -128.0, yend: 128.0
        zstart: -128.0, zend: 128.0
            TR: 6.81 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 
degrees
       nframes: 1
       PhEncDir: UNKNOWN
       FieldStrength: 0.000000
ras xform present
    xform info: x_r =  -1.0000, y_r =  -0.0000, z_r =  -0.0000, c_r =    -0.6003
              : x_a =   0.0000, y_a =   1.0000, z_a =   0.0000, c_a =    17.5182
              : x_s =  -0.0000, y_s =  -0.0000, z_s =   1.0000, c_s =    16.7755
Orientation   : LAS
Primary Slice Direction: axial

voxel to ras transform:
               -1.2000  -0.0000  -0.0000   101.3997
                0.0000   1.0000   0.0000  -110.4818
               -0.0000  -0.0000   1.0000  -111.2245
                0.0000   0.0000   0.0000     1.0000

voxel-to-ras determinant -1.2

ras to voxel transform:
               -0.8333  -0.0000  -0.0000    84.4998
               -0.0000   1.0000  -0.0000   110.4818
               -0.0000  -0.0000   1.0000   111.2245
               -0.0000  -0.0000  -0.0000     1.0000
niiRead(): detected input as 64 bit double, reading in as 32 bit float
Volume information for ants_Warped.nii.gz
          type: nii
    dimensions: 170 x 256 x 256
   voxel sizes: 1.200000, 1.000000, 1.000000
          type: FLOAT (3)
           fov: 204.000
           dof: 1
        xstart: -102.0, xend: 102.0
        ystart: -128.0, yend: 128.0
        zstart: -128.0, zend: 128.0
            TR: 0.00 msec, TE: 0.00 msec, TI: 0.00 msec, flip angle: 0.00 
degrees
       nframes: 1
       PhEncDir: UNKNOWN
       FieldStrength: 0.000000
ras xform present
    xform info: x_r =  -1.0000, y_r =  -0.0000, z_r =   0.0000, c_r =    -0.6003
              : x_a =  -0.0000, y_a =   1.0000, z_a =  -0.0000, c_a =    17.5182
              : x_s =   0.0000, y_s =   0.0000, z_s =   1.0000, c_s =    16.7755
Orientation   : LAS
Primary Slice Direction: axial

voxel to ras transform:
               -1.2000  -0.0000   0.0000   101.3997
               -0.0000   1.0000  -0.0000  -110.4818
                0.0000   0.0000   1.0000  -111.2245
                0.0000   0.0000   0.0000     1.0000

voxel-to-ras determinant -1.2

ras to voxel transform:
               -0.8333  -0.0000  -0.0000    84.4998
               -0.0000   1.0000  -0.0000   110.4818
               -0.0000  -0.0000   1.0000   111.2245
               -0.0000  -0.0000  -0.0000     1.0000
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