Nicole: CitriSolve works somewhat better than other d-Limonene xylene substitutes because it is a hybrid. It contains d-Limonene at 70% (or 50% according with other recipes) + quaternary amnine mixture (or aliphatic hydrocarbons in another recipe) at 25% (or greater %) and 2-butoxyethanol at 5%. This 2-butoxyethanol is a suspected carcinogen with a TWA of only 25 ppm. CitriSolve is described as corrosive to the eyes. It costs 2.31 times more than Xylene and I personally would not use it. René J.
--- On Wed, 10/8/08, kbowden <[EMAIL PROTECTED]> wrote: From: kbowden <[EMAIL PROTECTED]> Subject: Re: [Histonet] citrisolve? To: "Patten, Nicole (NIH/NIAAA) [F]" <[EMAIL PROTECTED]> Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, October 8, 2008, 5:37 PM I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using it our Environmental Health and Safety said pour it down the drain, but as time goes by and with more regulations now I have to collect it and have it removed with hazardous waste. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Patten, Nicole (NIH/NIAAA) [F] wrote: > Does anyone have any experience using Citrisolve as a Xylene alternative > to deparaffinize tissue sections? Do I use it just like Xylene for the > same amount of time? Can it be reused or should I use new Citrisolve > each time? How do I dispose of it? > > > > Any help at all would be much appreciated. Thanks! > > > > Nicole J. Patten > Post-Baccalaureate Fellow/IRTA > National Institutes of Health > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet