Are you filtering the solutions before immersing the slides? The sodium chloride is a saturated solution. Rena Fail -------------- Original message from Jane M Flinn <[EMAIL PROTECTED]>: --------------
We are running a congo red stain and have sodium chloride crystals precipataing > onto the slide. Does anyone know why and how we can fix this? jane > > "Life is short - make haste to be kind" > > Dr. Jane Flinn > Director, Undergraduate Neuroscience Program > George Mason University, 3F5 > 4400 University Dr. > Fairfax, VA 22030 > Phone: 703-993-4107 > Fax: 703-993-1359 > > > ----- Original Message ----- > From: kbowden > Date: Wednesday, October 8, 2008 5:37 pm > Subject: Re: [Histonet] citrisolve? > > > I have used Citrisolve for serveral years. I don't think anything > > compares to Xylene, but I do think this comes very close. I > > change it > > about once a week. It pretty much deparaffinizes in the same > > amount of > > time. Disposal is dependent on the regulations in your area. > > When I > > started using it our Environmental Health and Safety said pour it > > down > > the drain, but as time goes by and with more regulations now I > > have to > > collect it and have it removed with hazardous waste. > > -- > > Karen Bowden > > Staff Research Associate II > > University of CA, San Diego > > Department of Orthopedics > > 9500 Gilman Dr. 0630 > > La Jolla, CA 92093-0630 > > 858-534-4655 voice > > 858-534-5304 fax > > > > > > CONFIDENTIALITY NOTICE: > > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, > > DISSEMINATION OR > > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS > > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT > > THE SENDER AND > > DELETE THE MATERIAL FROM ANY COMPUTER. > > > > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > > Does anyone have any experience using Citrisolve as a Xylene > > alternative> to deparaffinize tissue sections? Do I use it just > > like Xylene for the > > > same amount of time? Can it be reused or should I use new Citrisolve > > > each time? How do I dispose of it? > > > > > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > > > > > Nicole J. Patten > > > Post-Baccalaureate Fellow/IRTA > > > National Institutes of Health > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet