We are running a congo red stain and have sodium chloride crystals precipataing onto the slide. Does anyone know why and how we can fix this? jane
"Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: kbowden <[EMAIL PROTECTED]> Date: Wednesday, October 8, 2008 5:37 pm Subject: Re: [Histonet] citrisolve? > I have used Citrisolve for serveral years. I don't think anything > compares to Xylene, but I do think this comes very close. I > change it > about once a week. It pretty much deparaffinizes in the same > amount of > time. Disposal is dependent on the regulations in your area. > When I > started using it our Environmental Health and Safety said pour it > down > the drain, but as time goes by and with more regulations now I > have to > collect it and have it removed with hazardous waste. > -- > Karen Bowden > Staff Research Associate II > University of CA, San Diego > Department of Orthopedics > 9500 Gilman Dr. 0630 > La Jolla, CA 92093-0630 > 858-534-4655 voice > 858-534-5304 fax > > > CONFIDENTIALITY NOTICE: > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, > DISSEMINATION OR > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT > THE SENDER AND > DELETE THE MATERIAL FROM ANY COMPUTER. > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > Does anyone have any experience using Citrisolve as a Xylene > alternative> to deparaffinize tissue sections? Do I use it just > like Xylene for the > > same amount of time? Can it be reused or should I use new Citrisolve > > each time? How do I dispose of it? > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > Nicole J. Patten > > Post-Baccalaureate Fellow/IRTA > > National Institutes of Health > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >
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