I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application?
I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
