I have had much better luck staining liver samples with Sudan Black B
rather than with Oil Red O. I suggest giving that a try.
Derek Papalegis HT (ASCP)
Senior Histology Technologist
Division of Laboratory Animal Medicine
Tufts University
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354
pam johnson wrote:
Michele,
I am having the same issues. I cannot get a good stain. I am getting alot of
non-specific staining. I am using the kit from PolyScientific. I am using mouse
tissue. I have never had so many problems with an Oil Red O. It is such a
simple stain.
-----Original Message-----
From: Michele Wich <[EMAIL PROTECTED]>
To: [email protected]
Sent: Mon, 17 Nov 2008 11:56 am
Subject: [Histonet] Oil Red O despair
I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application?
I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.
Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.
Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.
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