One of the main conditions that may prevent an adequate Oil Red O stain is the saturation of the dye. It can take one or more weeks for the Oil Red O dye to reach saturation in the solvent.
We have two bottles of Oil Red O solution prepared. We always prepare another batch when the first is emptied so that one is "ripening" while the older solution is being used. (Oh gee - did that make sense?) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Michele Wich Sent: Tuesday, 18 November 2008 4:57 AM To: [email protected] Subject: [Histonet] Oil Red O despair I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
