I didn't mean that the propylene glycol and glycerin jelly were
combined - just wanted to clear that up. The protocol uses both but
for different things.
Andi Grantham
At 09:12 AM 11/18/2008, Andrea Grantham wrote:
I get many projects here that require frozen liver sections and Oil
Red O staining. Some years ago I was having problems with the
staining and someone on histonet suggested the PolyScientific R & D
Oil Red O Stain Kit. I got the kit and have been using it ever
since. The stain works great - usually I have livers loaded with
lipid - and the slides come out clean. The kit uses proplyene glycol
and glycerin jelly to mount the coverslips. I do use one part of the
ORO protocol from Freida Carson's book - I fix the slides in 37-40%
(page 152, second edition).
Andi Grantham
At 04:49 PM 11/17/2008, Amos Brooks wrote:
Michele,
You could try 0.5% Oil Red O in 60% triethylphosphate. That works really
well here. I've also used a formulation I found in Humanson, the same amount
of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is
much cleaner than the ethanol. (There was a precipitate on the slide.) I
can't avouch for isopropyl as I used ethanol, but it is good to know there's
an alternative in case we run out of triethylphosphate.
Amos Brooks
Message: 10
Date: Mon, 17 Nov 2008 11:56:45 -0600
From: "Michele Wich" <[EMAIL PROTECTED]>
Subject: [Histonet] Oil Red O despair
To: <[email protected]>
Message-ID:
<[EMAIL PROTECTED]>
Content-Type: text/plain; charset="us-ascii"
I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application?
I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.
Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.
Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: [EMAIL PROTECTED]) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: [EMAIL PROTECTED]) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
