Please tell us what chromogen stains and counterstains you are using, and the type of mounting medium for the coverslip. This will help us try to answer your question.

Jan Shivers
UMN VDL

----- Original Message ----- From: "shazana hilda" <hilda_1...@yahoo.com>
To: <histonet@lists.utsouthwestern.edu>
Sent: Monday, February 02, 2009 8:10 PM
Subject: [Histonet] query regarding IHC slide


Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it?

Any response are greatly appreciated.


Regards.

Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1...@yahoo.com



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