I'm using DAB chromogen from LabVision and counterstained with Harris Hematoxylin from Sigma .Then mounted using Cytoseal XYL from Richard-Allan Scientific.
Thank You. Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Phone no: +6013-921 2002 Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] ________________________________ From: Jan Shivers <[email protected]> To: shazana hilda <[email protected]>; [email protected] Sent: Tuesday, February 3, 2009 11:12:09 PM Subject: Re: [Histonet] query regarding IHC slide Please tell us what chromogen stains and counterstains you are using, and the type of mounting medium for the coverslip. This will help us try to answer your question. Jan Shivers UMN VDL ----- Original Message ----- From: "shazana hilda" <[email protected]> To: <[email protected]> Sent: Monday, February 02, 2009 8:10 PM Subject: [Histonet] query regarding IHC slide > Dear All, > Is anyone has experience with faded IHC-slide stained? I've done my IHC > stained on few markers and after 3-4 months I viewed the slide and it seems > that the stain became faded.Does anyone has opinion regarding this matter and > how to troubleshoot it? > > Any response are greatly appreciated. > > > Regards. > > Shazana Hilda ShamsuddinUniversiti Sains Malaysia, > Off. no: +609- 766 4440 > Fax no: +609- 765 3370 > Email: [email protected] > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
