I'm using DAB Plus chromogen from LabVision and counterstained with Harris Hematoxylin from Sigma .Then mounted using Cytoseal XYL from Richard-Allan Scientific.I'm dehydrated the slide through graded of alcohol then cleared in xylene prior to mounting. After done the IHC I've stored the slide in slide box at RT (about 27degrees).
Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] ________________________________ From: "[email protected]" <[email protected]> To: shazana hilda <[email protected]>; histonet forum <[email protected]> Sent: Wednesday, February 4, 2009 11:37:17 AM Subject: Re: [Histonet] query regarding IHC slide What mounting media are you using? Are you dehydrating? How are you storing the slides? What DAB are you using (ie: source)? -----Original Message----- From: shazana hilda <[email protected]> Date: Tue, 03 Feb 2009 19:23:16 To: McMahon, Loralee A<[email protected]>; histonet forum<[email protected]> Subject: Re: [Histonet] query regarding IHC slide Dear Loralee, Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem. Thank You. Shazana Hilda ShamsuddinUniversiti Sains Malaysia Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] ________________________________ From: "McMahon, Loralee A" <[email protected]> To: shazana hilda <[email protected]> Sent: Tuesday, February 3, 2009 11:47:06 PM Subject: RE: [Histonet] query regarding IHC slide [Histonet] query regarding IHC slide Are you using DAB or AEC. AEC fades and DAB does not. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: [email protected] on behalf of shazana hilda Sent: Mon 2/2/2009 9:10 PM To: [email protected] Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
