Dear Loralee, Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem.
Thank You. Shazana Hilda ShamsuddinUniversiti Sains Malaysia Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1...@yahoo.com ________________________________ From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> To: shazana hilda <hilda_1...@yahoo.com> Sent: Tuesday, February 3, 2009 11:47:06 PM Subject: RE: [Histonet] query regarding IHC slide [Histonet] query regarding IHC slide Are you using DAB or AEC. AEC fades and DAB does not. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of shazana hilda Sent: Mon 2/2/2009 9:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1...@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet