Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). rené J.
--- On Wed, 3/4/09, Perry, Margaret <margaret.pe...@sdstate.edu> wrote: From: Perry, Margaret <margaret.pe...@sdstate.edu> Subject: [Histonet] problem eyes To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
