Good question! I'd like to know that, too! But with any size specimen,
please!
--On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello
<vapat...@yahoo.com> wrote:
Really? Liquid laundry soap? Does this help with all wrinkles in large
specimens?
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Wed, 3/4/09, Rene J Buesa <rjbu...@yahoo.com> wrote:
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>, "Perry, Margaret"
<margaret.pe...@sdstate.edu> Date: Wednesday, March 4, 2009, 9:14 PM
Add a few drops (4-5) of liquid
detergent, but not dishwasher detergent (because it will
dissolve the paraffin).
rené J.
--- On Wed, 3/4/09, Perry, Margaret <margaret.pe...@sdstate.edu>
wrote:
From: Perry, Margaret <margaret.pe...@sdstate.edu>
Subject: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Date: Wednesday, March 4, 2009, 4:04 PM
Please help! We have been trying to cut whole eyes
from a pig. They need to be
nice enough for a publication. We are having the
devil of a time because no
matter what we do there are wrinkles. If we turn the
waterbath up to stretch
things out the retina detaches. Do any of you have
suggestions? We uses
Surgipath EM400 for embedding paraffin and and their
infiltration medium. Is
this to soft? The lowest we can turn our waterbath is 38
degrees C.
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
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