thx. I did notice another anti-rat CD31 from Abcam (they have 41 types of anti-CD31). only mentioned works on Frozen section.
How's BD bioscience CD31 work on IHC-Frozen and paraffin sections (animals were fixed with PFA)? 2009-03-07 TF 发件人: Chiriboga, Luis 发送时间: 2009-03-07 00:16:34 收件人: ti...@foxmail.com; Colleen Forster 抄送: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题: RE: [Histonet] CD31 on rat brain For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with..... -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF 发件人: Colleen Forster 发送时间: 2009-03-06 00:57:29 收件人: tifei 抄送: iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题: Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > 发件人: iskaliora > 发送时间: 2009-03-05 18:49:11 > 收件人: histonet > 抄送: ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ > 主题: [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C᾽ (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskali...@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
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