Hello,

I really have some real links to real pictures of real relevance of the real histonet list. Really promised.

http://img12.imageshack.us/img12/8513/ts0402162049.jpg
http://img13.imageshack.us/img13/6514/ts0402162104.jpg

So, has ever anyone experienced sth. like this?
My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all.

I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea?

Thanks,

V. Neubert

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