Hi V. That my friend is the result of a air bubble on the section. If it had been unremoved xylene the hematoxylin would have been absent as well. Cheers. Greg
Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "V. Neubert" <histonet.nos...@vneubert.com> 4/2/2009 1:12:18 PM >>> Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet