I have seen something (rarely) like this. We always assume that a few bubbles developed that impeded the flow of one or more critical reagents to the tissue underlying the bubble. Somehow, the impediment vanished later in staining to allow for the Hematoxylin counterstain to take. It would only take the omission of one reagent to halt the immunochemical reaction in that area. Just a thought.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of V. Neubert Sent: Thursday, April 02, 2009 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Strange circles in IHC slides Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet