I forgot to mention: dirt Happy Friday
Paula On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell <[email protected]> wrote: > Orangutan testicle macro section and alligator jawbones, not my best work, > very humbling, after 52 years in the business. > Shirley > > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Morken, Timothy > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had > to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte > that was nearly microscopic. I'll just say it took a LOT of thick sections > too face down to it without actually cutting through it. > > > Open the floodgates.... > > Tim Morken > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol > methacrylate (gma) it is much harder and would give better sections, we > have done zebra fish and several other harder tissues including calcified > bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > [email protected] > > > > > From: [email protected] > > To: [email protected]; [email protected] > > Date: Sat, 3 Jan 2015 23:15:33 +0000 > > Subject: RE: [Histonet] cutting honey bees > > CC: > > > > I sectioned and stained honey bee and yellow jacket stingers years ago. > They wanted to show the difference between the stingers. I wasn't sure > what to do so I processed and handled like everything else. I was able to > get some good sections. I put 6 stingers in each block and cut several > sections figuring there should be at least one good stinger in each block > and it worked. > > Roberta Horner > > Penn State University > > Animal Diagnostic Lab > > ________________________________________ > > From: [email protected] > > [[email protected]] on behalf of Douglas Gregg > > [[email protected]] > > Sent: Saturday, January 03, 2015 6:08 PM > > To: [email protected] > > Subject: [Histonet] cutting honey bees > > > > Has anyone had experience embedding and cutting honey bees. I am sure > > there are some issues with the harder exoskeleton. Would that have to > > be dissected away first. I am considering helping a student with a > > science fair project on bees. > > > > Douglas Gregg > > Veterianary pathologist > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
