Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates.... Tim Morken -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 [email protected] > From: [email protected] > To: [email protected]; [email protected] > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They > wanted to show the difference between the stingers. I wasn't sure what to do > so I processed and handled like everything else. I was able to get some good > sections. I put 6 stingers in each block and cut several sections figuring > there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: [email protected] > [[email protected]] on behalf of Douglas Gregg > [[email protected]] > Sent: Saturday, January 03, 2015 6:08 PM > To: [email protected] > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
