Hi, Strand-specific RT-PCR would be the easiest way. In that case, the RT step is performed using a primer specific to one or the other transcript.
Sébastien On Sat, Mar 27, 2010 at 11:32 AM, Maryam <[email protected]> wrote: > Hi All > We have 2 genes that are antisense to each other. I was wondering how we > can distinguish the expression of these two genes. > Thanks in advance. > > > Maryam > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
