Hi Reza, trimerization for sure will increase the enzyme's activity. It is hard to say if the catalytic domain alone will show any activity. This will a lot depend on where you separate the domains and of course all domains influence each other. I would not suggest just to place the cut somewhere and hope that (at least some) activity might remain.
If you are lucky, someone already has done this fragment analysis and published the results. If not, and you need this information, it will be up to you to perform these experiments. hope that helps Wo. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
