Hello Guys I have a trivial query, I have a protein stock 1000 µg per ml. Now I would like to load on each well 0.25, 0.5, 1, 2, 4, 8, 16 and 32 µgs. For 0.25 µg, 0.25 µl from stock + 0.25 µl 2x sample buffer , total 0.5 µl load on first lane. If I want to load higher amount like 16 µg and 32 µgs more volume to be loaded but I could load maximum16 µl in each well. In that case, should I use C1 V1 = C2 V2 formula, 1000 µg * ? = 16 µg * 8 µl V1 = 0.128 micro liter from the stock, 7.8 ul milli q water, to that add 8 µl 2x sample buffer and load 16 ul to the well, Am I right doing things here?????
Thank you in advance -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
