In article <mailman.70.1325623817.3721.meth...@net.bio.net>, hroyc...@nmsu.edu says... > > Since you say you have few questions, I am assuming that you are quite > certain about your composition. PBS stands for "Phosphate buffered > saline" so it is NaCl, not KCl. Usually we use Na-salts for the > phosphate combination. Potassium phosphate and -biphosphate may be > used, but for all practical purposes it is Na- that is preferred since > lot of compounds do not go well with K+
Actually no - PBS (in the strict sense) is designd to resemble the extracellular fluid, and hence contains both Na and K: mM g/l NaCl 137 8.01 KCl 2.7 0.20 Na2HPO4 ? 2 H2O 10 1.78 KH2PO4 2.0 0.27 Depending on application, Mg and Ca may also be added (e.g., Dulbecco's PBS). Even closer to extracellular fluid is the Krebs-Henseleit solution, which also contains glucose and bicarbonate. Btw, if one wants to work with intracellular enzymes, PBS should not be used, and replaced with a high K, low Na medium! _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods