Thanks for the reference Professor Polly, very helpful.

Alex Marshall
MSci Student 
University College London
alexander....@ucl.ac.uk

On Saturday, 28 March 2015 09:13:51 UTC, pdpolly wrote:
>
> Phylogenetic PCA has many unusual properties, and does not remove the 
> effects of phylogeny.  Yes, it is the case that the variance of the scores 
> on the first phylogenetic principal component can be higher than the second 
> PC.  We recently had a paper in the Hystrix morphometrics volumes about the 
> properties of pPCA and what it does and does not do.
>
> Polly, P. D., A. M. Lawing, A.-C. Fabre, and A. Goswami. 2013. 
> Phylogenetic principal components analysis and geometric morphometrics. 
> *Hystrix*, 24: 1-9
>
> Paper is available open access from the publisher or from here: 
> http://mypage.iu.edu/~pdpolly/Publications.html 
>
> P. David Polly
> Geological Sciences
> Adjunct, Anthropology and Biology
> Indiana University
> 1001 E. 10th Street
> Bloomington, IN  47405-1405
> pdp...@indiana.edu <javascript:>
> +1 (812) 855-7994
> http://mypage.iu.edu/~pdpolly/
>  
> On 27 Mar 2015, at 7:49 AM, Alex Marshall <alpm...@googlemail.com 
> <javascript:>> wrote:
>
> Hello everyone,
> I'm a MSci student new to morphometrics and this group. I'm studying 
> morphological integration in squamate crania and one of the things I'd like 
> to do is an Evolutionary PCA of all my species, accounting for phylogeny 
> and allometry.
>
> I think I do this correctly but in the PCA results the PCs are not ranked 
> by proportion of variance e.g. PC5 has greater % variance that PCs 3 & 4. 
> Is this normal?
>
> To conduct the evolutionary PCA I created independant contrasts of all my 
> shape data, regressed centroid size on shape and conducted PCA on the 
> residuals. After this I applied the resultant PC scores to another PCA of 
> my original data.
>
> Would anyone kindly confirm if this is the right way to do it?
>
> Many thanks,
>
> Alex Marshall
> MSci Student 
> University College London
> alexander....@ucl.ac.uk <javascript:>
>
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